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In vitro effects of glyphosate-based herbicides and related adjuvants on primary culture of hemocytes from Haliotis tuberculata.

Authors
  • Mottier, Antoine1
  • Serpentini, Antoine1
  • Dallas, Lorna2
  • James, Adèle1
  • Lebel, Jean-Marc1
  • Costil, Katherine3
  • 1 Normandie Université, Université de Caen Normandie, F-14032, Caen, France; BOREA (Biologie des Organismes et des Ecosystèmes Aquatiques), UCN, MNHN, UPMC, CNRS-7208, IRD-207, IBFA, Université de Caen Normandie, Esplanade de la Paix, CS 14032, 14032, Caen, Cedex 5, France. , (France)
  • 2 School of Biological Sciences, University of Plymouth, Drake Circus, Plymouth, PL4 8AA, UK.
  • 3 Normandie Université, Université de Caen Normandie, F-14032, Caen, France; BOREA (Biologie des Organismes et des Ecosystèmes Aquatiques), UCN, MNHN, UPMC, CNRS-7208, IRD-207, IBFA, Université de Caen Normandie, Esplanade de la Paix, CS 14032, 14032, Caen, Cedex 5, France. Electronic address: [email protected] , (France)
Type
Published Article
Journal
Fish & Shellfish Immunology
Publisher
Elsevier
Publication Date
Feb 29, 2020
Volume
100
Pages
1–8
Identifiers
DOI: 10.1016/j.fsi.2020.02.058
PMID: 32119910
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Glyphosate-based herbicides are among the most produced and widely-used herbicides. Studies have shown that commercial formulations and adjuvants may be more toxic to non-target organisms than the active ingredients alone, but the mechanisms of action of these chemicals remain unclear. The aim of this study was to investigate the in vitro effects of glyphosate, a commercial formulation and adjuvant alone using primary culture of hemocytes from the European abalone Haliotis tuberculata, a commonly farmed shellfish. Glyphosate was found to have negligible effects on viability, phagocytic activities and lysosome stability even with very high doses (i.e. 100 mg L-1). By contrast, greater effects on viability were observed for the commercial formulation and adjuvant alone, with EC50 values of 41.42 mg L-1 and 1.85 mg L-1, respectively. These results demonstrate that the toxic sublethal effects (i.e. phagocytic activity and destabilization of lysosomal membranes) of formulated glyphosate came from adjuvants and suggest they may be related to cell and organelle membrane destabilization. Copyright © 2020. Published by Elsevier Ltd.

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