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In vitro effects of glyphosate and Roundup on Sertoli cell physiology.

Authors
  • Gorga, Agostina1
  • Rindone, Gustavo Marcelo1
  • Centola, Cecilia Lucia1
  • Sobarzo, Cristian2
  • Pellizzari, Eliana Herminia1
  • Camberos, María Del Carmen1
  • Cigorraga, Selva Beatriz1
  • Riera, Maria Fernanda1
  • Galardo, Maria Noel1
  • Meroni, Silvina Beatriz3
  • 1 CONICET-FEI-División de Endocrinología, Centro de Investigaciones Endocrinológicas "Dr César Bergadá", Hospital de Niños Ricardo Gutiérrez, Argentina. , (Argentina)
  • 2 Facultad de Medicina, UBA, Instituto de Investigaciones Biomédicas (INBIOMED), Argentina. , (Argentina)
  • 3 CONICET-FEI-División de Endocrinología, Centro de Investigaciones Endocrinológicas "Dr César Bergadá", Hospital de Niños Ricardo Gutiérrez, Argentina. Electronic address: [email protected] , (Argentina)
Type
Published Article
Journal
Toxicology in vitro : an international journal published in association with BIBRA
Publication Date
Feb 01, 2020
Volume
62
Pages
104682–104682
Identifiers
DOI: 10.1016/j.tiv.2019.104682
PMID: 31626902
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Roundup (R), a formulation that contains glyphosate (G) as the active ingredient, is a commonly used nonselective herbicide that has been proposed to affect male fertility. It is well known that an adequate Sertoli cell function is essential to maintain germ cell development. The aim of the present study was to analyze whether G and R are able to affect Sertoli cell functions, such as energy metabolism and blood-testis barrier (BTB) integrity. Sertoli cell cultures from 20-day-old rats were exposed to 10 and 100 ppm of G or R, doses which do not decrease cell viability. Neither G nor R caused impairment in lactate production or fatty acid oxidation. G and R decreased Transepithelial Electrical Resistance, which indicates the establishment of a Sertoli cell junction barrier. However, neither G nor R modified the expression of claudin11, ZO1 and occludin, proteins that constitute the BTB. Analysis of cellular distribution of claudin11 by immunofluorescence showed that G and R induced a delocalization of the signal from membrane to the cytoplasm. The results suggest that G and R could alter an important function of Sertoli cell such as BTB integrity and thus they could compromise the normal development of spermatogenesis. Copyright © 2019 Elsevier Ltd. All rights reserved.

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