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In vitro differentiation of Trypanosoma cruzi epimastigotes into metacyclic trypomastigotes using a biphasic medium

Authors
  • Rodríguez Durán, Jessica1
  • Muñoz-Calderón, Arturo1
  • Gómez, Karina Andrea1
  • Potenza, Mariana1
  • 1 Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres” (INGEBI-CONICET), Ciudad Autónoma de Buenos Aires, Buenos Aires, C1428ADN, Argentina
Type
Published Article
Journal
STAR Protocols
Publisher
Elsevier
Publication Date
Sep 01, 2021
Volume
2
Issue
3
Identifiers
DOI: 10.1016/j.xpro.2021.100703
PMID: 34505085
PMCID: PMC8417395
Source
PubMed Central
Keywords
Disciplines
  • Protocol
License
Unknown

Abstract

The pathogen Trypanosoma cruzi differentiates from epimastigotes (E) into infective metacyclic trypomastigotes (MTs) to invade the mammalian cell. This process, called metacyclogenesis, is mimicked in vitro by nutrient starvation or incubation with minimal media. Here, we describe an alternative protocol for metacyclogenesis by incubating E forms in a biphasic medium supplemented with human blood. Although time consuming, this procedure yields fully differentiated MTs without the presence of intermediate forms, even for cultures that have been maintained as E for years.

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