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In vitro differentiation ofPieris brassicae imaginal wing discs: Effects and metabolism of ecdysone and ecdysterone.

Authors
  • Blais, Catherine1
  • Lafont, René1
  • 1 Laboratoire de Zoologie, Ecole Normale Supérieure, 46, rue d'Ulm, F-75230, Paris Cedex 05, France. , (France)
Type
Published Article
Journal
Wilhelm Roux's archives of developmental biology
Publication Date
Feb 01, 1980
Volume
188
Issue
1
Pages
27–36
Identifiers
DOI: 10.1007/BF00848607
PMID: 28305152
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Imaginal wing discs ofPieris brassicae can be cultured in vitro for extended periods. Their ultrastructural development was investigated after culture in the presence of various concentrations of ecdysone and ecdysterone. When ecdysone or low concentrations of ecdysterone (2×10-7 M) were added to culture media, larval discs secreted a pupal cuticle and they subsequently differentiated scales; prepupal discs completed their imaginal development. With a higher concentration of ecdysterone (2×10-6 M), all discs produced abundant but fragmentary cuticular material.Prepupal discs were able to metabolize both hormones in vitro. Ecdysterone was mainly converted into a polar compound detectable after a short period of incubation. Ecdysone was transformed, at a slower rate, forming a polar compound and 26-hydroxyecdysone but no ecdysterone.

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