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In vitro differentiation of multiple shoot clumps from intact seedlings of switchgrass

Authors
  • Gupta, S. Dutta1
  • Conger, B. V.2
  • 1 Indian Institute of Technology, Department of Agricultural and Food Engineering, Kharagpur, 721302, India , Kharagpur
  • 2 University of Tennessee, Department of Plant and Soil Science, Knoxville, Tennessee, 37901-1071 , Knoxville
Type
Published Article
Journal
In Vitro Cellular & Developmental Biology - Plant
Publisher
Springer-Verlag
Publication Date
Jul 01, 1998
Volume
34
Issue
3
Pages
196–202
Identifiers
DOI: 10.1007/BF02822708
Source
Springer Nature
Keywords
License
Yellow

Abstract

An efficient and reproduciblein vitro culture system has been developed for regeneration of multiple shoot clumps from intact seedlings of both lowland and upland cultivars of switchgrass (Panicum virgatum L.). The multiple shoots were induced on Murashige and Skoog medium supplemented with various combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 1-phenyl-3-(1,2,3-thiadiazol-5YL)-urea (thidiazuron or TDZ). Maximum response was obtained with 4.5 μM 2,4-D and 18.2 μM TDZ. These shoots proliferated and rooted efficiently on MS medium without growth regulators. The developmental pattern of the multiple shoots indicated their origin from the enlarged shoot apex via proliferation of axillary buds and subsequent reprogramming of shoot meristems followed by secondary differentiation of adventitious shoots The simplicity of the protocol and direct production of multiple shoots make this a potential system that is highly attractive and amenable for microprojectile-mediated gene transfer.

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