Affordable Access

In vitro destabilization of plant viruses and cDNA synthesis.

Authors
  • Wyatt, S D
  • Druffel, K
  • Berger, P H
Type
Published Article
Journal
Journal of Virological Methods
Publisher
Elsevier
Publication Date
Oct 01, 1993
Volume
44
Issue
2-3
Pages
211–220
Identifiers
PMID: 8263116
Source
Medline
License
Unknown

Abstract

DNA copies of a wide range of RNA viruses can be made by the direct addition of appropriately treated, purified virus particles to a reverse transcription reaction. Therefore, many problems associated with RNA isolation can be circumvented. Virus particles can be sufficiently destabilized by adjustments of salt content, buffer, pH or by the use of physical force supplied by a freeze/thaw cycle so that RNA in sufficient quantity and physical condition is available for the synthesis of in some cases, full length cDNAs. cDNAs have been made of viruses in the bromo-, poty-, carla-, ilar-, potex-, tobra and tobamovirus groups. Reported here are experiments with cowpea chlorotic mottle virus and bean common mosaic virus.

Report this publication

Statistics

Seen <100 times