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In vitro Cultivation of the Entomogenous Nematode Filipjevimermis leipsandra.

Authors
Type
Published Article
Journal
Journal of nematology
0022-300X
Publication Date
Volume
14
Issue
1
Pages
126–131
Identifiers
PMID: 19295686
Source
Medline
Keywords
  • Diabrotica Balteata
  • Banded Cucumber Beetle
  • Mermithid

Abstract

The mermithid nematode, Filipjevimermis leipsandra, was successfully cultivated to the preadult stage in Schneider's Drosophila medium supplemented with 20% fetal bovine serum. Upon transfer to a solid substrate the preadults continued to develop into ovipositing adult females. Four molts were observed. The first molt occurred in the egg. The second occurred after 6-8 days in culture during which the very thin cuticle was shed completely. The third molt occurred after 18-20 days in culture; the cuticle was retained by the third-stage nematode. This stage was considered comparable to the preadult stage that emerges from host larva, Diabrotica spp. The fourth molt occurred within 12 days after the preadult was transferred from the liquid medium to a solid substrate. Adult females began ovipositing viable eggs 1-3 days after the final molt.

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