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In vitro capacitation of goat spermatozoa.

Authors
  • Anand, S R
  • Atreja, S K
  • Chauhan, M S
  • Behl, R
Type
Published Article
Journal
Indian journal of experimental biology
Publication Date
Nov 01, 1989
Volume
27
Issue
11
Pages
921–924
Identifiers
PMID: 2559890
Source
Medline
License
Unknown

Abstract

Goat epididymal and ejaculated spermatozoa were incubated in Krebs-Ringer bicarbonate buffer containing pyruvate and lactate as energy source. A 3 hr incubation for epididymal and 4 hr for ejaculated spermatozoa was required for the capacitation and acrosome reaction to take place. Calcium is an essential requirement which was needed for motility maintenance/activation and for the initiation of acrosome reaction. A 2-fold increase in cAMP content was measured over 3 hr period of incubation of goat epididymal spermatozoa which was not seen when calcium ions were either omitted or chelated with EGTA. There is thus a definite involvement of Ca2+ ions and cAMP in capacitation and acrosome reaction of goat spermatozoa.

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