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In vitro antiviral activity of Griffithsin against porcine epidemic diarrhea virus

Authors
  • Li, Lan
  • Yu, Xiaoming
  • Zhang, Haoming
  • Cheng, Haiwei
  • Hou, Liting
  • Zheng, Qisheng
  • Hou, Jibo
Type
Published Article
Journal
Virus Genes
Publisher
Springer-Verlag
Publication Date
Jan 14, 2019
Volume
55
Issue
2
Pages
174–181
Identifiers
DOI: 10.1007/s11262-019-01633-7
Source
Springer Nature
Keywords
License
Yellow

Abstract

Griffithsin is a lectin with potent antiviral activity against enveloped viruses. The objective of this study was to assess Griffithsin’s inhibitory effect on porcine epidemic diarrhea virus (PEDV). The results showed that Griffithsin reduced PEDV infection of Vero cells by approximately 82.8%. Moreover, using time-of-addition assays and RT-qPCR, we found that delayed addition of Griffithsin had a weaker inhibitory effect on PEDV than earlier treatment. The mechanism of Griffithsin’s action against PEDV involved both preventing viral attachment to host cells and disrupting cell-to-cell transmission; its dual mode of action distinguished Griffithsin from most other antiviral drugs. In conclusion, Griffithsin was identified as a potent PEDV inhibitor and may represent a candidate drug for preventing PEDV infection.

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