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Vitreous composition modification after transpalpebral electrical stimulation of the eye: Biochemical analysis.

Authors
  • Colombo, Leonardo1
  • Caretti, Anna2
  • Dei Cas, Michele3
  • Luciano, Francesco2
  • Romano, Dario1
  • Paroni, Rita4
  • Patelli, Fabio1
  • Ghidoni, Riccardo2
  • Rossetti, Luca1
  • 1 Eye Clinic, ASST Santi Paolo e Carlo Hospital, Università degli Studi di Milano, Milan, Italy. , (Italy)
  • 2 Biochemistry and Molecular Biology Laboratory, Department of Health Sciences, Università degli Studi di Milano, Milan, Italy. , (Italy)
  • 3 Clinical Biochemistry and Mass Spectrometry Laboratory, Department of Health Sciences, Università degli Studi di Milano, Milan, Italy. Electronic address: [email protected] , (Italy)
  • 4 Clinical Biochemistry and Mass Spectrometry Laboratory, Department of Health Sciences, Università degli Studi di Milano, Milan, Italy. , (Italy)
Type
Published Article
Journal
Experimental Eye Research
Publisher
Elsevier
Publication Date
Jun 01, 2021
Volume
207
Pages
108601–108601
Identifiers
DOI: 10.1016/j.exer.2021.108601
PMID: 33910035
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Electrical stimulation (ES) of the eye represents a therapeutic approach in various clinical applications ranging from retinal dystrophies, age-related macular degeneration, retinal artery occlusion and nonarteritic ischemic optic neuropathy. In clinical practice, ES of the eye is mainly performed with a transcorneal or transpalpebral approach. These procedures are non-invasive and well-tolerated by the patients, reporting only minimal and transient adverse events, while serious adverse effects were not observed. Despite the growing literature on animal models, only clinical parameters have been investigated in humans and few data are available about biochemical changes induced by ES of the eye. The purpose of this study is to investigate the possible mechanism that regulates the beneficial effects of ES on retinal cells function and survival in humans. 28 patients undergoing pars plana vitrectomy (PPV) for idiopathic epiretinal membrane (iERM) were randomly divided in two groups: 13 patients were treated with transpalpebral ES before surgery and 15 underwent surgery with no prior treatment. Vitreous samples were collected for biochemical analysis during PPV. ES treatment leads to a reduction in the vitreous expression of both proinflammatory cytokines, namely IL-6 and IL-8, and proinflammatory lipid mediators, such as lysophosphatidylcholine. Indeed, we observed a 70% decrease of lysophosphatidylcholine 18:0, which has been proven to exert the greatest proinflammatory activities among the lysophosphatidylcholine class. The content of triglycerides is also affected and significantly decreased following ES application. The vitreous composition of patients undergoing PPV for iERM displays significant changes following ES treatment. Proinflammatory cytokines and bioactive lipid mediators expression decreases, suggesting an overall anti-inflammatory potential of ES. The investigation of the mechanism by which this treatment alters the retinal neurons leading to good outcomes is essential for supporting ES therapeutic application in various types of retinal diseases. Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.

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