Affordable Access

deepdyve-link deepdyve-link
Publisher Website

Visualizing of the cellular uptake and intracellular trafficking of exosomes by live-cell microscopy.

Authors
Type
Published Article
Journal
Journal of Cellular Biochemistry
1097-4644
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
111
Issue
2
Pages
488–496
Identifiers
DOI: 10.1002/jcb.22733
PMID: 20533300
Source
Medline
License
Unknown

Abstract

Cells release exosomes to transfer various molecules to other cells. Exosomes are involved in a number of physiological and pathological processes. They are emerging great potential utility for diseases diagnosis and treatment recently. However, the internalization and intracellular trafficking of exosomes have not been described clearly. In this work, exosomes were isolated from the culture medium of PC12 cells, labeled by lipophilic dye and amino-reactive fluorophore, incubated with resting PC12 cells. The results of live-cell microscopy indicated that exosomes were internalized through endocytosis pathway, trapped in vesicles, and transported to perinuclear region. Particle tracking fluorescent vesicles suggested that the active transport of exosomes may be mediated by cytoskeleton. The proteins on exosome membrane were found to be released from exosomes and trapped in lysosome. The inverted transport of lipophilic dye from perinuclear region to cell peripheries was revealed, possibly caused by recycling of the exosome lipids. This study provides new sight into the mechanisms of exosome uptake and intracellular fate.

Statistics

Seen <100 times