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Visualization of serum albumin on electrophoretic gels using the specific ligand bilirubin.

Authors
  • Khan, M M
  • Muzammil, S
  • Kumar, Y
  • Tayyab, S
Type
Published Article
Journal
Journal of Biochemical and Biophysical Methods
Publisher
Elsevier
Publication Date
Sep 24, 1998
Volume
37
Issue
1-2
Pages
47–52
Identifiers
PMID: 9825299
Source
Medline
License
Unknown

Abstract

Serum albumin, when incubated with bilirubin prior to electrophoresis, was visualized as a yellow-colored band during the electrophoretic run and did not require any staining. Furthermore, free bilirubin served as a tracking dye. A minimum of 20 micrograms of protein was detected very well by this method. The formation of a bilirubin-albumin complex did not affect the electrophoretic mobility as the protein complexed with bilirubin as well as free albumin moved with the same mobility. Only a single protein band was visualized by this method after electrophoresis of human plasma. The method is simple, less time-consuming and may be used in identifying bilirubin-binding proteins in various biological samples.

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