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Visualization of individual DNA molecules in solution by light microscopy: DAPI staining method.

Authors
  • Morikawa, K
  • Yanagida, M
Type
Published Article
Journal
Journal of biochemistry
Publication Date
Feb 01, 1981
Volume
89
Issue
2
Pages
693–696
Identifiers
PMID: 6165713
Source
Medline
License
Unknown

Abstract

A method was developed to visualize individual DNA molecules in solution under a fluorescent microscope connected to a highly sensitive video camera. DNA stained with a fluorescent dye, DAPI, revealed thin extended filaments, thicker filaments and rapidly transforming folded structures dependent upon the solution conditions. Structural transitions were observed and recorded as video images. Our observations indicated the possibility that DNA has the ability of supercoiling itself. This DAPI staining method will have wide possible application in the study of DNA and chromatin.

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