Although many disinfectants are commercially available in the veterinary field, information on the virucidal effects of disinfectants against equine group A rotavirus (RVA) is limited. We evaluated the performance of commercially available disinfectants against equine RVA. Chlorine- and iodine-based disinfectants showed virucidal effects, but these were reduced by the presence of organic matter. Glutaraldehyde had a virucidal effect regardless of the presence of organic matter, but the effect was reduced by low temperature or short reaction time, or both. Benzalkonium chloride had the greatest virucidal effect among the three quaternary ammonium compounds examined, but its effect was reduced by the presence of organic matter or by low temperature or a short reaction time. These findings will be useful for preventing the spread of equine RVA infection.