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VIIaAT complexes, procoagulant phospholipids, and thrombin generation during postprandial lipemia.

Authors
  • Silveira, A1
  • Carlo, A2
  • Adam, M2
  • McLeod, O1
  • Lundman, P3
  • Boquist, S4
  • Woodhams, B J5
  • Hamsten, A1, 4
  • 1 Cardiovascular Medicine Unit, Department of Medicine Solna and Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden. , (Sweden)
  • 2 Diagnostica Stago, Gennevilliers, France. , (France)
  • 3 Department of Clinical Sciences, Division of Cardiovascular Medicine, Danderyd University Hospital, Karolinska Institutet, Stockholm, Sweden. , (Sweden)
  • 4 Department of Cardiology, Karolinska University Hospital Solna, Stockholm, Sweden. , (Sweden)
  • 5 HaemaCon Ltd, Bromley, UK.
Type
Published Article
Journal
International journal of laboratory hematology
Publication Date
Jun 01, 2018
Volume
40
Issue
3
Pages
251–257
Identifiers
DOI: 10.1111/ijlh.12773
PMID: 29356352
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Factor VII activation occurs postprandially. A proportion of activated factor VII (VIIa) circulates in complex with antithrombin (VIIaAT). Our primary objective was to assess the effects of postprandial lipemia on circulating VIIaAT, procoagulant phospholipid (PPL) activity, and thrombin generation. Plasma samples from postmyocardial infarction patients (n = 40) and controls (n = 39) were taken before and at 3 and 6 hours during a standardized oral fat tolerance test (OFTT). Fasting PPL activity measurements were also made in a second cohort of 108 postinfarction patients and 109 controls. VIIaAT was analyzed with the Asserachrom VIIaAT ELISA, PPL activity with the STA-Procoag-PPL kit, and thrombin generation with calibrated automated thrombogram with PRP-Reagent as trigger (all Diagnostica Stago products). Postprandially, VIIaAT increased in all samples without significant case-control differences in the overall response during the OFTT. Thrombin generation measures peak height and velocity, and PPL activity, were marginally affected by the test meal in the controls. Levels of all patient baseline measures were significantly different from controls, indicating a more hypercoagulable state, and these differences were maintained throughout the OFTT. Fasting samples from cases showed higher PPL activity than control samples. Viewing VIIaAT quantitation as a surrogate for TF activity measurement, postprandial increase in VIIaAT may reflect a mechanism that adds to the cardiovascular risk associated with postprandial lipemia. On the other hand, the impact of postprandial lipemia on PPL activity and thrombin generation seems to be minor. © 2018 John Wiley & Sons Ltd.

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