Affordable Access

deepdyve-link
Publisher Website

Viability of Descemet membrane endothelial keratoplasty grafts folded in the eye bank

Authors
  • Lohmeier, John1
  • Christy, Jason1
  • Chiang, Eric2
  • Barnes, Kali2
  • Cai, Stephanie2
  • Chen, Conan2
  • Subramanya, Anshul2
  • Chaurasia, Akash2
  • Rosen, Allison2
  • Vora, Parth2
  • Durr, Nicholas J.2
  • Allen, Robert2
  • Eghrari, Allen Omid3
  • 1 Rocky Mountain Lions Eye Bank, Denver, Colorado
  • 2 Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland
  • 3 Wilmer Eye Institute at Johns Hopkins, Baltimore, Maryland
Type
Published Article
Journal
Cornea
Publication Date
Nov 01, 2018
Volume
37
Issue
11
Pages
1474–1477
Identifiers
DOI: 10.1097/ICO.0000000000001711
PMID: 30095494
PMCID: PMC6173616
Source
PubMed Central
Keywords
License
Unknown

Abstract

Purpose Preloaded, tri-folded grafts in Descemet Membrane Endothelial Keratoplasty require transfer of the tri-folding process from the cornea transplant surgeon to the eye bank technician. We sought to assess if tri-folding may be safely conducted by an eye bank technician with cell loss comparable to standard peeling and lifting. Methods A total of ten grafts were stained, peeled and transferred directly onto a bed of Calcein-AM and Amvisc Plus by an eye bank technician. Five grafts were removed and stained as a scroll and five grafts tri-folded with the endothelium in before transfer. Photographs were acquired with an inverted fluorescence microscope and image segmentation was performed. A t-test was conducted to compare for difference in endothelial cell loss across groups. Results Mean cell loss in the scroll group was 18.5% [95% confidence interval (CI): 15.2-21.9%] compared to 7.6% the tri-folded group (95% CI: 1.7-13.5%). A two-tailed t-test indicated decreased cell loss in the tri-folded group (p=0.013). Conclusion Despite additional manipulation of the graft, tri-folding of Descemet membrane and endothelium may be performed by an eye bank technician without significantly increased cell loss relative to graft preparation as a scroll.

Report this publication

Statistics

Seen <100 times