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[The viability assessment of ethanol-producing yeast by computer-aided fluorescence microscopy].

Authors
Type
Published Article
Journal
Mikrobiologiia
Publication Date
Volume
75
Issue
2
Pages
193–200
Identifiers
PMID: 16758867
Source
Medline

Abstract

Vital staining of the ethanol-producing yeast Saccharomyces cerevisiae with ethidium bromide and DAPI allows intact and damaged cells to be differentiated by fluorescence microscopy. A computer image analysis procedure has been developed for the automatic determination of the relative number of damaged cells using ImageJ software (National Institute of Health, United States; http://rsb.info.nih.gov./ij/). A good correlation has been found between the viability rates determined by the plate count method and the relative numbers of intact cells assessed by the developed procedure in the dry-yeast preparations rehydrated under various conditions.

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