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Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein.

Authors
  • Beckers, C J
  • Block, M R
  • Glick, B S
  • Rothman, J E
  • Balch, W E
Type
Published Article
Journal
Nature
Publication Date
Jun 01, 1989
Volume
339
Issue
6223
Pages
397–398
Identifiers
PMID: 2542798
Source
Medline
License
Unknown

Abstract

An N-ethylmaleimide-sensitive fusion protein (NSF) has been purified on the basis of its ability to catalyse vesicular transport within the Golgi stack. We report here that this same protein is required for transport from the endoplasmic reticulum to the Golgi stack in semi-intact cells. This transport process is inhibited by a monoclonal antibody against NSF. Furthermore, pretreatment of semi-intact cells with N-ethylmaleimide, a sulphydryl alkylating reagent, inhibits transport. Addition of highly purified NSF largely restores transport from endoplasmic reticulum to Golgi. These results suggest that NSF is a general component of the transport machinery required for membrane fusion at multiple stages of the secretory pathway.

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