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The variation in frequency with which markers are transduced by phage P1 is primarily a result of discrimination during recombination.

Authors
Type
Published Article
Journal
Molecular & general genetics : MGG
Publication Date
Volume
180
Issue
3
Pages
585–589
Identifiers
PMID: 7007821
Source
Medline
License
Unknown

Abstract

The efficiency of recovery of P1 transductants is marker dependent and normally varies over a 25-fold range. UV irradiation of either transducing lysates for recipient cells results in a selective stimulation of the transduction of markers which are normally transduced poorly. As a result the range in frequency of transduction is reduced to about 3-fold and resembles the gene frequency distribution expected in the donor cells. We conclude that P1 transducing lysates are likely to contain a random sample of donor DNA but that the recombination system of the recipient cell exhibits a preference for the DNA of some regions over that of others. Damage to DNA presumably overrides this specificity.

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