Affordable Access

Variation between mouse major urinary protein genes isolated from a single inbred line.

Authors
Type
Published Article
Journal
Gene
0378-1119
Publisher
Elsevier
Publication Date
Volume
18
Issue
3
Pages
221–230
Identifiers
PMID: 6290325
Source
Medline
License
Unknown

Abstract

We describe ten Charon 4A genomic DNA clones from BALB/c mice which include at least seven different major urinary protein (MUP) genes. We have established the orientation of all seven sequences, and have placed six of them in precise register by means of restriction site maps and Southern blot hybridization with cloned cDNA sequences. Four of the seven genomic sequences (family I sequences) form hybrids with six independent cDNA clones that have a high thermal stability and hybridize more strongly with mRNA from three inbred mouse lines. Hybrids between the remaining three genomic sequences and the cDNA clones have a lower thermal stability and hybridize less strongly with mRNA from the three inbred lines. Homologies between different cloned sequences extend over as much as 15 kb. No clone contains parts of two MUP genes, and no homology has been detected between the 3' flanking region of one MUP gene and the 5' flanking region of another.

Statistics

Seen <100 times