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Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods.

Authors
  • Berzaghi, Rodrigo
  • da Silva, Silvia Helena Marques
  • de Camargo, Zoilo Pires
Type
Published Article
Journal
Journal of clinical microbiology
Publication Date
Jan 01, 2005
Volume
43
Issue
1
Pages
491–493
Identifiers
PMID: 15635024
Source
Medline
License
Unknown

Abstract

The main objectives of this study were to obtain clones of Paracoccidioides brasiliensis by two methods (micromanipulation and plating assay) and to determine if the secretion of the 43-kDa glycoprotein (gp43) is dependent on the clonal culture. The results show that the secretion of gp43 is not dependent on clonal cultures. Clones that originally were secretors of this molecule, after subculturing, lost this characteristic; on the other hand, clones that originally did not secrete gp43 began to secrete gp43 after subculturing.

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