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Variability of the response of human vaginal Lactobacillus crispatus to 17β-estradiol

Authors
  • Clabaut, Maximilien1
  • Boukerb, Amine M.1
  • Mlouka, Amine Ben2
  • Suet, Amandine3
  • Tahrioui, Ali1
  • Verdon, Julien4
  • Barreau, Magalie1
  • Maillot, Olivier1
  • Le Tirant, Agathe5
  • Karsybayeva, Madina6
  • Kremser, Coralie7
  • Redziniak, Gérard8
  • Duclairoir-Poc, Cécile1
  • Pichon, Chantal3
  • Hardouin, Julie2
  • Cosette, Pascal2
  • Chevalier, Sylvie1
  • Feuilloley, Marc G. J.1
  • 1 Rouen Normandie Université, 55 rue Saint-Germain, Evreux, 27000, France , Evreux (France)
  • 2 Proteomic Platform PISSARO University of Rouen Normandy, Mont-Saint-Aignan, France , Mont-Saint-Aignan (France)
  • 3 UPR4301 French National Centre for Scientific Research, Orléans, France , Orléans (France)
  • 4 Université de Poitiers, Poitiers, France , Poitiers (France)
  • 5 Seqens Cosmetics, Porcheville, France , Porcheville (France)
  • 6 Remedials Laboratory, Paris, France , Paris (France)
  • 7 GymoPharm, Longjumeau, France , Longjumeau (France)
  • 8 Cosmetic Inventions, Antony, France , Antony (France)
Type
Published Article
Journal
Scientific Reports
Publisher
Springer Nature
Publication Date
Jun 01, 2021
Volume
11
Issue
1
Identifiers
DOI: 10.1038/s41598-021-91017-5
Source
Springer Nature
License
Green

Abstract

We previously showed that the physiological concentration of 17β-estradiol in the vaginal environment is sufficient to affect the membrane dynamics and adhesion phenotype of the Lactobacillus crispatus strain CIP104459. However, L. crispatus is a heterogeneous species. Here, we investigated the effect of 17β-estradiol on the recently isolated L. crispatus vaginal strain V4, related to a cluster distant from CIP104459 and at the limit of being a different subspecies. Grown in the same medium, the two strains expressed a highly similar pool of proteins. However, in contrast to CIP104459, L. crispatus V4 showed high aggregation potential and 17β-estradiol promoted this phenotype. This effect was associated with large changes in cell-surface polarity and Lewis acid/base properties. In addition, we observed no effect on the membrane dynamics, contrary to CIP104459. These results can be explained by differences in the properties and organization of the S layer between the two strains. However, as for CIP104459, 17β-estradiol increased biosurfactant production of L. crispatus V4 and their adhesion to vaginal cells. This suggests that 17β-estradiol agonists would be valuable tools to favor a stable re-implantation of L. crispatus in the vaginal mucosa.

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