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Validation of gene expression profiles of candidate genes using low density array in peripheral blood of tobacco consuming head and neck cancer patients and auto/taxi drivers with preneoplastic lesions.

Authors
  • Hasan, Feza1
  • Yadav, Vinay2
  • Katiyar, Tridiv1
  • Yadav, Sanjay2
  • Pandey, Rahul3
  • Mehrotra, Divya3
  • Hadi, Rahat4
  • Singh, Sudhir3
  • Bhatt, Madan L B3
  • Parmar, Devendra5
  • 1 Developmental Toxicology Laboratory, System Toxicology & Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (IITR), Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, Lucknow 226001, U.P., India; Babu Banarsi Das University, Faizabad Road, Lucknow 226028, U.P., India. , (India)
  • 2 Developmental Toxicology Laboratory, System Toxicology & Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (IITR), Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, Lucknow 226001, U.P., India. , (India)
  • 3 Department of Radiotherapy, Department of Oral and Maxillofacial Surgery, King George's Medical University, Lucknow 226003, U.P., India. , (India)
  • 4 Department of Radiation Oncology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Gomti Nagar, Lucknow 226010, U.P., India. , (India)
  • 5 Developmental Toxicology Laboratory, System Toxicology & Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (IITR), Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, Lucknow 226001, U.P., India. Electronic address: [email protected] , (India)
Type
Published Article
Journal
Genomics
Publisher
Elsevier
Publication Date
Jan 01, 2020
Volume
112
Issue
1
Pages
513–519
Identifiers
DOI: 10.1016/j.ygeno.2019.04.003
PMID: 30951801
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

TaqMan Low-Density Array (TLDA) based Real-Time PCR (RT-PCR) of selected genes showed increased expression of polycyclic aromatic hydrocarbons (PAHs) metabolizing cytochrome P450s (CYPs), glutathione S-transferases (GSTs) and associated transcription factors in biopsy and peripheral blood samples isolated from head and neck squamous cell carcinoma (HNSCC) patients when compared to the controls. The genes involved in DNA repair, signal transduction pathway, EMT pathway, apoptosis, and cell adhesion/motility were found to be altered in both peripheral blood and biopsy samples of HNSCC patients. Transcription profiles in blood isolated from auto/taxi drivers, with pre-neoplastic lesions and history of tobacco use, also showed similar alterations. The present TLDA data thus demonstrates that low-density array of selected genes in peripheral blood has the potential to be used as a surrogate for providing insight into cancer progression pathways and possibly as an early biomarker for monitoring tobacco induced HNSCC. Copyright © 2019. Published by Elsevier Inc.

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