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Validation and evaluation of a common biomarker in human cancers sera protein detected by a monoclonal antibody UNIVmAb

Authors
  • Manjunath, D.1
  • Kumaraswamy, Sunil B.1
  • Venkatakrishniah, Shashidhar Aladhi2
  • Appaiah, Hitesh Nidumanda1
  • Thomas, Anil1
  • Banerjee, Shib D.3
  • 1 Preethi Center of Oncology, Vattavyalil Cancer Trust, Mysore, Karnataka, India , Mysore (India)
  • 2 Logic and Clue Diagnostics, Mysore, India , Mysore (India)
  • 3 Tuft University School of Medicine, Department of Anatomy and Cellular Biology, Boston, MA, USA , Boston (United States)
Type
Published Article
Journal
BMC Research Notes
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Nov 14, 2019
Volume
12
Issue
1
Identifiers
DOI: 10.1186/s13104-019-4780-4
Source
Springer Nature
Keywords
License
Green

Abstract

ObjectiveManagement and diagnosis of multiple human cancers remains a challenge and search for a common biomarker is still debatable. In this manuscript we have evaluated the use of monoclonal antibody UNIVmAb, to detect the protein (H11) as a common biomarker for all cancers irrespective of the grade and origin. We have shown by both ELISA and Western Blot that the H11 protein, is a unique hyaluronan binding protein that has not been detected earlier. H11 protein was fractionated in an anion exchange column followed by cibacron blue gel exclusion chromatography. Hyaluronan binding H11 protein reacted with Monoclonal antibody UNIVmAb and b-HA inspite of b-Hyaluronan (biotinylated Hyaluronan) interaction and HA-Oligo (Hyaluronan oligosaccharides) competition from various grades of Human cancers sera.ResultsELISA, Western blot and b-Hyaluronan interactions clearly showed an over-expression of UNIVmAb reacted H11 protein in all fifty cancer’s sera when compared with seventy normal sera. UNIVmAb reactive H11 protein can be used as a common biomarker. We believe, UNIVmAb detected H11 protein, is a unique hyaluronan binding protein, that can be used as a common biomarker for all cancers.

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