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Vacuolar pH in radish cotyledonal mesophyll cells.

Authors
Type
Published Article
Journal
Planta
Publication Date
Volume
172
Issue
4
Pages
563–565
Identifiers
DOI: 10.1007/BF00393875
PMID: 24226078
Source
Medline
License
Unknown

Abstract

The vacuolar pH in cotyledonal mesophyll cells from radish (Raphanus sativus L. var. sativus) seedlings was determined from vacuoles, isolated from protoplasts through osmotic shock, by means of measurement of vacuole extracts with a pH meter and the "methylamine method", and gave mean pH values of 6.28 and 6.26, respectively. Direct in situ measurements of the vacuolar pH from intact leaf tissue were recorded with pH-sensitive microelectrodes and gave a mean value of 6.0. The results are discussed with respect to possible erroneous pH measurements and the vacuolar location of specific anabolic reactions.

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