Affordable Access

Vaccination of sheep against Fasciola hepatica with homologous fatty acid binding proteins.

Authors
  • Ramajo, V
  • Oleaga, A
  • Casanueva, P
  • Hillyer, G V
  • Muro, A
Type
Published Article
Journal
Veterinary Parasitology
Publisher
Elsevier
Publication Date
May 09, 2001
Volume
97
Issue
1
Pages
35–46
Identifiers
PMID: 11337125
Source
Medline
License
Unknown

Abstract

The current study was designed to test the immunoprophylactic properties of native (nFh12) and recombinant (rFh15) antigens from Fasciola hepatica in sheep subsequently infected with the fluke. Thirty lambs were divided into six groups according to various patterns of immunisation and times of infection and necropsy. The antigens were emulsified in Freund's adjuvant. Levels of specific anti-nFh12 and anti-rFh15 antibodies rose rapidly by 2 weeks after the first immunisation and were always significantly higher in immunised-infected sheep than in control-infected sheep. On completion of the trial there was no difference in fluke burden between groups vaccinated with either of the antigens and non-immunised controls. However, worm size and faecal egg counts were significantly diminished in the sheep vaccinated with either of the antigens, suggesting an anti-fecundity effect. This is the first report of experimental vaccination of sheep against F. hepatica with purified native and recombinant antigens related to fatty acid binding proteins.

Report this publication

Statistics

Seen <100 times