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Vaccination with human papillomavirus-18 E1 protein plus α-galactosyl-ceramide induces CD8+ cytotoxic response and impairs the growth of E1-expressing tumors.

Authors
  • Amador-Molina, Alfredo1
  • Trejo-Moreno, Cesar1
  • Romero-Rodríguez, Damaris2
  • Sada-Ovalle, Isabel2
  • Pérez-Cárdenas, Enrique1
  • Lamoyi, Edmundo3
  • Moreno, José4
  • Lizano, Marcela5
  • 1 Unidad de Investigación Biomédica en Cáncer, Instituto Nacional de Cancerología, Av. San Fernando No. 22, Col. Sección XVI, Tlalpan, Ciudad de México 14080, Mexico. , (Mexico)
  • 2 Laboratorio de Inmunología Integrativa, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Calzada de Tlalpan 4502, Tlalpan, Ciudad de México 14080, Mexico. , (Mexico)
  • 3 Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México 04510, Mexico. , (Mexico)
  • 4 Dirección de Investigación, Hospital Juárez de México, Ciudad de México 07760, Mexico. , (Mexico)
  • 5 Unidad de Investigación Biomédica en Cáncer, Instituto Nacional de Cancerología, Av. San Fernando No. 22, Col. Sección XVI, Tlalpan, Ciudad de México 14080, Mexico; Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México 04510, Mexico. Electronic address: [email protected] , (Mexico)
Type
Published Article
Journal
Vaccine
Publication Date
Feb 21, 2019
Volume
37
Issue
9
Pages
1219–1228
Identifiers
DOI: 10.1016/j.vaccine.2018.12.036
PMID: 30704821
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

CD8+ T cell-mediated immune response plays a major role in the clearance of virus-infected cells, including human papillomavirus (HPV). The effective treatment of women with normal cytology but persistent high risk-HPV infection or with low-grade intraepithelial lesions could take advantage of novel strategies based on vaccination with viral immunological targets with a wide spectrum of cross-protection. The helicase E1, expressed early during viral replication in HPV infection, is among the most conserved papillomavirus proteins, which makes it a good vaccine candidate. In the present study, we examined E1-specific CD8+ T cell and NK immune responses in a mouse model with α-galactosylceramide (α-GalCer) as an adjuvant. We found that mice immunized with E1 combined with α-GalCer elicited an E1-specific CD8+ T and NK cell cytotoxic responses, which correlated with growth inhibition of grafted melanoma B16-F0 cells expressing E1, both in prophylactic and therapeutic protocols. Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.

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