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UV-induced extracellular factor from human fibroblasts communicates the UV response to nonirradiated cells.

Authors
  • Schorpp, M
  • Mallick, U
  • Rahmsdorf, H J
  • Herrlich, P
Type
Published Article
Journal
Cell
Publisher
Elsevier
Publication Date
Jul 01, 1984
Volume
37
Issue
3
Pages
861–868
Identifiers
PMID: 6744414
Source
Medline
License
Unknown

Abstract

Ultraviolet light enhances the synthesis of at least eight abundant proteins in human fibroblasts within 2 hr. These proteins are identical with those induced by the tumor promoter TPA. The inducing signal is generated by DNA damage, as these proteins are induced by lower doses of UV in fibroblasts from patients with Cockayne's syndrome or Xeroderma pigmentosum. In the supernatant of UV-treated cells, a heat-labile ammonium sulfate precipitable factor of more than 10 kd (EPIF) was detected which, upon transfer to nonirradiated cells, mimicked UV in the UV-induced synthesis of gene products. The response to UV, TPA, or EPIF was inhibited by fluocinolone acetonide, but not by retinoic acid, protease inhibitors, or superoxide dismutase.

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