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Uterotonic Effects of Aqueous and Methanolic Extracts of Lannea acida in Wistar Rats: An In Vitro Study.

Authors
  • Ngadjui, Esther1, 2
  • Kouam, Jibril Yves3
  • Fozin, Georges Romeo Bonsou3
  • Momo, Aimé Césaire Tetsatsi3
  • Deeh, Patrick Brice Defo3
  • Wankeu-Nya, Modeste4
  • Nguelefack, Telesphore Benoit3
  • Watcho, Pierre3
  • 1 Research Unit of Animal Physiology and Phytopharmacology, University of Dschang, Box 67, Dschang, Cameroon. [email protected] , (Cameroon)
  • 2 Department of Physiological and Biochemical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Box 67, Dschang, Cameroon. [email protected] , (Cameroon)
  • 3 Research Unit of Animal Physiology and Phytopharmacology, University of Dschang, Box 67, Dschang, Cameroon. , (Cameroon)
  • 4 Department of Animal Organisms Biology, University of Douala, P.O. BOX, 24157, Douala, Cameroon. , (Cameroon)
Type
Published Article
Journal
Reproductive Sciences
Publisher
SAGE Publications
Publication Date
Sep 01, 2021
Volume
28
Issue
9
Pages
2448–2457
Identifiers
DOI: 10.1007/s43032-021-00465-x
PMID: 33475981
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Lannea acida (Anacardiaceae), commonly called Kikié in the Noun division (West-Cameroon), is a tree whose bark is used locally to facilitate delivery. This study was aimed at evaluating the in vitro uterotonic effects of aqueous and methanol extracts of L. acida in Wistar rats. Uterine strips isolated from rats pretreated with 5 μg estradiol (48 h) were mounted in a single-organ bath containing aerated and thermostated De Jalon solution (37 °C). After equilibration, non-cumulative effects of L. acida extracts were recorded after application. The effect of the methanol extract (the most active extract) was monitored in the presence of atosiban (a competitive antagonist of oxytocin receptors), atropine (a specific type 3 muscarinic receptor antagonist), nifedipine (an L-type calcium channel antagonist), and 2-Aminoethoxydiphenyl borate (2-ADB, a specific antagonist of inositol 1,4,5-triphosphate receptors type 1), and in calcium-free medium containing EGTA to elucidate its mechanism of action. L. acida induced uterine contraction in a concentration-dependent manner with the methanol extract (1.506 ± 0.032 gf) being the most effective. Administration of atosiban (2 μmol/L) and atropine (1 μmol/L) reduced the contractile effect of L. acida. Complete inhibition was observed with nifedipine, 2-APB, and calcium-free medium containing EGTA. These results suggest that L. acida possesses uterotonic effects mediated through oxytocin receptors with mobilization of extracellular calcium. © 2021. Society for Reproductive Investigation.

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