The aim of this study is to evaluate human thermal comfort in different green area settings in the city of Florence by using the Universal Thermal Climate Index (UTCI). Field measurements of air temperature, solar radiation, relative humidity, wind speed and black globe thermometer were collected during hot summer days in various parts of Cascine Park, the biggest urban park in Florence (Italy). UTCI was evaluated over different surfaces (asphalt, gravel and grass) completely exposed to the sun or shaded by a large lime tree (Tilia × europaea). The results showed strong differences in UTCI values depending on the exposure to tree shade, while no significant difference was found among ground-cover materials when all surfaces were equally exposed to solar radiation. Future studies are needed to investigate the microclimatic effects of different tree species on UTCI.