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Usutu virus growth in human cell lines: induction of and sensitivity to type I and III interferons.

Authors
  • Scagnolari, Carolina
  • Caputo, Beniamino
  • Trombetti, Simona
  • Cacciotti, Giulia
  • Soldà, Annalisa
  • Spano, Lucia
  • Villari, Paolo
  • della Torre, Alessandra
  • Nowotny, Norbert
  • Antonelli, Guido
Type
Published Article
Journal
Journal of General Virology
Publisher
Microbiology Society
Publication Date
Apr 01, 2013
Volume
94
Issue
Pt 4
Pages
789–795
Identifiers
DOI: 10.1099/vir.0.046433-0
PMID: 23255619
Source
Medline
License
Unknown

Abstract

The mechanisms of Usutu virus (USUV) pathogenesis are largely unknown. The aim of this study was to evaluate the sensitivity of USUV to interferon (IFN) and the capacity of USUV to stimulate IFN production. Initial experiments were conducted to characterize the susceptibility of human cell lines to USUV infection and to evaluate the single-growth cycle replication curve of USUV. Results indicate that USUV is able to infect a variety of human cell lines, completing the replication cycle in Hep-2 and Vero cells within 48 h. Pre-treatment of cells with types I and III IFNs significantly inhibited the replication of USUV. However, the inhibitory effects of IFNs were considerably less if IFN was added after viral infection had been initiated. Also, USUV weakly induced types I and III IFNs.

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