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Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide

Authors
  • Lohr, Christian1
  • Beiersdorfer, Antonia1
  • Fischer, Timo1
  • Hirnet, Daniela1
  • Rotermund, Natalie1
  • Sauer, Jessica1
  • Schulz, Kristina1
  • Gee, Christine E.2
  • 1 Division of Neurophysiology, University of Hamburg, Hamburg , (Germany)
  • 2 Institute of Synaptic Physiology, University Medical Center Hamburg-Eppendorf, Hamburg , (Germany)
Type
Published Article
Journal
Frontiers in Cellular Neuroscience
Publisher
Frontiers Media SA
Publication Date
Jun 11, 2021
Volume
15
Identifiers
DOI: 10.3389/fncel.2021.690147
Source
Frontiers
Keywords
Disciplines
  • Cellular Neuroscience
  • Mini Review
License
Green

Abstract

Ca2+ imaging is the most frequently used technique to study glial cell physiology. While chemical Ca2+ indicators served to visualize and measure changes in glial cell cytosolic Ca2+ concentration for several decades, genetically encoded Ca2+ indicators (GECIs) have become state of the art in recent years. Great improvements have been made since the development of the first GECI and a large number of GECIs with different physical properties exist, rendering it difficult to select the optimal Ca2+ indicator. This review discusses some of the most frequently used GECIs and their suitability for glial cell research.

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