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The use of turbulent flow chromatography and the isocratic focusing effect to achieve on-line cleanup and concentration of neat biological samples for low-level metabolite analysis.

Authors
  • Herman, J L
Type
Published Article
Journal
Rapid communications in mass spectrometry : RCM
Publication Date
Jan 01, 2005
Volume
19
Issue
5
Pages
696–700
Identifiers
PMID: 15700230
Source
Medline
License
Unknown

Abstract

The use of turbulent flow chromatography in conjunction with column switching isocratic focusing was used to perform on-line sample cleanup and concentration of neat rat plasma for the identification of low-level metabolites. The concentration was achieved by focusing multiple injections, which were cleaned by a turbulent flow column, onto an analytical column prior to elution into the mass spectrometer. In addition, the first application of turbulent flow chromatography for on-line sample cleanup of neat bile samples is reported. The on-line cleanup and concentration method extracts and concentrates a sample 20-fold in 1 h, and is completely automated.

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