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Use of a tissue sectioner to expose internal structures of biological samples for scanning electron microscopy.

Authors
  • Brown, M F
  • Brotzman, H G
  • Kinden, D A
Type
Published Article
Journal
Stain technology
Publication Date
Sep 01, 1976
Volume
51
Issue
5
Pages
267–270
Identifiers
PMID: 1033616
Source
Medline
License
Unknown

Abstract

A procedure yielding sections of unembedded biological samples for observation by scanning electron microscopy is described. Sections of samples, fixed and hardened in OsO4, were obtained in quantity with a tissue sectioner. Subsequent treatments to osmium-coat cut surfaces were employed prior to critical point drying. The procedure yields cleanly cut surfaces through cells and cytoplasmic organelles which are retained in their normal position. Sections of apple leaf and mouse kidney are illustrated. Sections can be readily cut in a desired plane with less structural damage than is typically encountered by other sectioning or dissection techniques.

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