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Use of tentoxin and nigericin to investigate the possible contribution of ΔpH to energy dissipation and the control of electron transport in spinach leaves

Authors
  • Quick, Paul
  • Scheibe, Renate
  • Stitt, Mark
Type
Published Article
Journal
Biochimica et Biophysica Acta (BBA) - Bioenergetics
Publisher
Elsevier
Publication Date
Jan 01, 1989
Volume
974
Issue
3
Pages
282–288
Identifiers
DOI: 10.1016/S0005-2728(89)80245-2
Source
Elsevier
Keywords
License
Unknown

Abstract

(a) Spinach leaf discs were floated overnight on various concentrations of tentoxin, an ATP synthase inhibitor, or nigericin, an uncoupler. They were then illuminated in saturating CO 2. (b) With tentoxin, the inhibition of photosynthesis was accompanied by lower ATP/ADP ratios and increased ‘energy’ quenching of chlorophyll fluorescence. There was a small increase in the reduction of the Photosystem II acceptor, Q A, as monitored by photochemical quenching of chlorophyll fluorescence. However, activation of NADP-malate dehydrogenase decreased, showing that the acceptor side of Photosystem I becomes more oxidised, (c) With nigericin, the inhibition of photosynthesis was accompanied by decreased ATP/ADP ratios, decreased energy quenching, Q A became more reduced than with tentoxin, and the acceptor side of Photosystem I also became more reduced, (d) The results are used to discuss the contribution of “energy” quenching to energy dissipation, and the operation of photosynthetic control in leaves.

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