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Use of the Hungate anaerobic technique in the isolation of phloroglucinol-negative mutants of Coprococcus species.

Authors
  • L A Thompson
  • D M Gates
  • W M Ingledew
  • G A Jones
Publication Date
Jan 01, 1976
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

The Hungate anaerobic technique was used with a standard procedure for bacterial mutagenesis employing N-methyl-N-nitro-N'-nitrosoguanidine to obtain mutants of an obligate anaerobe. Three mutant strains were derived from a Coprococcus sp., strain Pe15, a rumen anaerobe capable of growing on phloroglucinol. The mutants did not grow on phloroglucinol but did degrade the compound in anaerobic washed-cell suspensions, producing the same end products in approximately the same proportions as the wild type. It was concluded that the mutants were blocked in a unique step or steps necessary for carbon skeleton or energy synthesis from phloroglucinol and not in formation of an enzyme involved in the pathway of phloroglucinol degradation.

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