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Use of peroxidase-conjugated antiglobulin as an alternative to immunofluorescence for the detection of antinuclear factor in serum

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Abstract

A method of detecting serum antinuclear factor by the use of antiglobulin conjugated with horseradish peroxidase has been compared with a standard procedure using a fluorescent label. The horseradish peroxidase conjugate was evaluated by block titration against positive serum and by its performance as a test reagent for screening and titrating sera. It is concluded that the horse-radish peroxidase method, in which an ordinary light microscope is employed, provides a satisfactory alternative to immunofluorescence for routine tests for antinuclear factor.

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