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The use of native fluorescence analysis of synovial fluid in the diagnosis of medial compartment disease in medium- and large-breed dogs.

Authors
  • Bilská, Kamila1
  • Šteffeková, Zuzana2
  • Birková, Anna2
  • Mareková, Mária2
  • Ledecký, Valent2
  • Hluchý, Marián2
  • Kisková, Terézia2
  • 1 Small Animal Clinic, Department of Surgery, Orthopaedics, Radiology and Reproduction, (Bilská, Ledecký, Hluchý), University of Veterinary Medicine and Pharmacy, Košice, SlovakiaDepartment of Chemistry, Biochemistry and Biophysics (Šteffeková), University of Veterinary Medicine and Pharmacy, Košice, SlovakiaDepartment of Medical and Clinical Biochemistry and LABMED, a.s., Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovakia (Birková, Mareková)Department of Animal Physiology, Institute of Biology and Ecology, Pavol Jozef Šafárik University, Košice, Slovakia (Kisková) [email protected] , (Slovakia)
  • 2 Small Animal Clinic, Department of Surgery, Orthopaedics, Radiology and Reproduction, (Bilská, Ledecký, Hluchý), University of Veterinary Medicine and Pharmacy, Košice, SlovakiaDepartment of Chemistry, Biochemistry and Biophysics (Šteffeková), University of Veterinary Medicine and Pharmacy, Košice, SlovakiaDepartment of Medical and Clinical Biochemistry and LABMED, a.s., Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovakia (Birková, Mareková)Department of Animal Physiology, Institute of Biology and Ecology, Pavol Jozef Šafárik University, Košice, Slovakia (Kisková). , (Slovakia)
Type
Published Article
Journal
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Publication Date
May 01, 2016
Volume
28
Issue
3
Pages
332–337
Identifiers
DOI: 10.1177/1040638716640312
PMID: 27016720
Source
Medline
Keywords
License
Unknown

Abstract

We assumed that proteins are most likely responsible for synovial fluid fluorescence and that changes detected in fluorescence intensity are most likely the result of changes in the concentration of fluorescent proteins. Synchronous fluorescent matrices from synovial fluid samples were measured in the excitation wavelength range of 200-350 nm using a luminescence spectrophotometer. The synchronous matrix of synovial fluid consists of 2 dominant fluorescent centers (F1 and F2) in the ultraviolet region. The fluorescence intensities of both centers were significantly higher in pathological samples, with p = 0.001 (a 59% increase of the median value) for the F1 center and p = 0.002 (a 52% increase of the median value) for the F2 center. Receiver operating characteristic analysis confirmed that synovial fluid autofluorescence is a significant predictor of medial compartment disease in dogs, with the area under the curve at 0.776 (F1) and 0.778 (F2). We did not detect any differences in the autofluorescence of synovial fluid between male and female, or any breed-based changes. No position changes of fluorescent centers were recorded in the synovial fluid in diseased dogs compared with healthy dogs. The synovial fluid metabolic fingerprint of canine patients with medial compartment disease differed from that of healthy dogs. Our study demonstrated the feasibility of synovial fluid fingerprinting to identify disease-specific profiles of synovial fluid metabolites.

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