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Use of a medium buffered with N-hydroxyethylpiperazine-N-ethanesulfonate (HEPES) in intracytoplasmic sperm injection procedures is detrimental to the outcome of in vitro fertilization.

Authors
  • Morgia, Francesco
  • Torti, Monica
  • Montigiani, Monica
  • Piscitelli, Claudio
  • Giallonardo, Annalise
  • Schimberni, Mauro
  • Giannini, Pierluigi
  • Sbracia, Marco
Type
Published Article
Journal
Fertility and Sterility
Publisher
Elsevier
Publication Date
May 01, 2006
Volume
85
Issue
5
Pages
1415–1419
Identifiers
PMID: 16600227
Source
Medline
License
Unknown

Abstract

Our study showed that the use of media buffered with HEPES, during the microinjection of sperm into the oocytes, is detrimental for IVF outcome and should be avoided.

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