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Use of MALDI-TOF MS (Bruker Daltonics) for identification of Mycobacterium species isolated directly from liquid medium.

Authors
  • Fernández-Esgueva, Marta1
  • Fernández-Simon, Rebeca2
  • Monforte-Cirac, María Luisa2
  • López-Calleja, Ana Isabel2
  • Fortuño, Blanca2
  • Viñuelas-Bayon, Jesús3
  • 1 Servicio de Microbiología, Hospital Universitario Miguel Servet. IIS Aragón, Zaragoza, Spain. Electronic address: [email protected] , (Spain)
  • 2 Servicio de Microbiología, Hospital Universitario Miguel Servet. IIS Aragón, Zaragoza, Spain. , (Spain)
  • 3 Servicio de Microbiología, Hospital Universitario Miguel Servet. IIS Aragón, Zaragoza, Spain; Grupo de estudio de las Infecciones por Micobacterias (GEIM), Sociedad Española de Microbiología y Enfermedades Infecciosas, Spain. , (Spain)
Type
Published Article
Journal
Enfermedades infecciosas y microbiologia clinica (English ed.)
Publication Date
May 01, 2021
Volume
39
Issue
5
Pages
241–243
Identifiers
DOI: 10.1016/j.eimc.2020.05.011
PMID: 32536597
Source
Medline
Keywords
Language
Spanish
License
Unknown

Abstract

The aim of this study was to describe the evaluation of the use of MALDI-TOF MS for the identification of non-tuberculous mycobacteria (NTM) and Mycobacterium tuberculosis directly from liquid MGIT cultures from January 2017 to December 2017. A total of 155 isolates (mainly respiratory) were analyzed by MALDI-TOF MS (Bruker Daltonics) directly from MGIT liquid medium with a previous extraction procedure. MALDI-TOF MS generated acceptable scores for 152 isolates (98.06%). Fifty isolates were identified as M. tuberculosis complex and the remaining 105 as NTM (M. abscessus subsp. abscessus, M. avium, M. celatum, M chelonae, M. chimaera, M. fortuitum, M. gordonae, M. intracellulare, M. kansasii, M. lentiflavum, M. mageritense, M. mucogenicum and M. xenopi). These results indicate that MALDI-TOF MS can be useful to identify mycobacteria directly from MGIT cultures and is an accurate, rapid and cost-effective system to be used as a routine method. Copyright © 2020 Elsevier España, S.L.U. and Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.

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