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The use of LipidGreen2 for visualization and quantification of intracellular Poly(3-hydroxybutyrate) in Cupriavidus necator

Authors
  • Kettner, Alexander
  • Griehl, Carola
Type
Published Article
Journal
Biochemistry and Biophysics Reports
Publisher
Elsevier
Publication Date
Sep 30, 2020
Volume
24
Identifiers
DOI: 10.1016/j.bbrep.2020.100819
PMID: 33024843
PMCID: PMC7528054
Source
PubMed Central
Keywords
License
Unknown

Abstract

• LipidGreen2 stains PHB selectively for spectroscopic determination and bioimaging. • 3DEEM and stability experiments help to identify optimal staining conditions. • Correction of inner filter effects using sample side scatter values improves linear correlation. • Strong linearity allows exact fluorescence-based PHB quantification in just 15 min.

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