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[Use of an immunoenzyme method on a solid-phase carrier for determining the tularemia antigen].

Authors
Type
Published Article
Journal
Zhurnal mikrobiologii, epidemiologii, i immunobiologii
Publication Date
Issue
5
Pages
88–92
Identifiers
PMID: 6741370
Source
Medline
License
Unknown

Abstract

The method of the highly sensitive (up to 10 ng/ml) and specific determination of soluble tularemia antigen, based on the use of "sandwich" type ELISA techniques, has been developed. The dependence of the specificity and sensitivity of the method on the degree of purification of antibodies and their peroxidase conjugates used in the assay has been studied. The study has revealed that the best results can be obtained with the use of purified IgG and its conjugate free of unbound peroxidase. Both foreign peroxidase preparations and type A enzyme manufactured in the USSR can be equally used as enzymatic labels.

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