Affordable Access

deepdyve-link
Publisher Website

Use of europium ions for SAD phasing of lysozyme at the Cu Kα wavelength.

Authors
  • Vijayakumar, Balakrishnan
  • Velmurugan, Devadasan
Type
Published Article
Journal
Acta Crystallographica Section F Structural Biology and Crystallization Communications
Publisher
International Union of Crystallography
Publication Date
Jan 01, 2013
Volume
69
Issue
Pt 1
Pages
20–24
Identifiers
DOI: 10.1107/S1744309112047562
PMID: 23295480
Source
Medline
License
Unknown

Abstract

Europium is shown to be a good anomalous scatterer in SAD phasing for solving the structure of biological macromolecules. The large value of the anomalous contribution of europium, f'' = 11.17 e(-), at the Cu Kα wavelength is an advantage in de novo phasing and automated model building. Tetragonal crystals of hen egg-white lysozyme (HEWL) incorporating europium(III) chloride (50 mM) were obtained which diffracted to a resolution of 2.3 Å at a wavelength of 1.54 Å (Cu Kα). The master data set (360° frames) was split and analyzed for anomalous signal-to-noise ratio, multiplicity, completeness, SAD phasing and automated building. The structure solution and model building of the split data sets were carried out using phenix.autosol and phenix.autobuild. The contributions of the Eu ions to SAD phasing using in-house data collection are discussed. This study revealed successful lysozyme phasing by SAD using laboratory-source data involving Eu ions, which are mainly coordinated by the side chains of Asn46, Asp52 and Asp101 together with some water molecules.

Report this publication

Statistics

Seen <100 times