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The use of electrospray ionization mass spectrometry to monitor RNA-ligand interactions.

Authors
  • Dremann, Danielle N1
  • Chow, Christine S2
  • 1 Wayne State University, Detroit, MI, United States. , (United States)
  • 2 Wayne State University, Detroit, MI, United States. Electronic address: [email protected] , (United States)
Type
Published Article
Journal
Methods in enzymology
Publication Date
Jan 01, 2019
Volume
623
Pages
315–337
Identifiers
DOI: 10.1016/bs.mie.2019.05.013
PMID: 31239052
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

RNAs are drawing increasing attention as potential therapeutic targets. A significant challenge in the RNA drug discovery process is identification of compounds that not only disrupt the natural functions of RNA by binding with high affinity, but also do so selectively. Assessing the binding mode of small molecules with RNA is important for understanding how they select their binding site and impart their mechanism of action. A number of complementary assays are often employed for analysis of the binding mode and to determine selectivity. One important technique that gives information about the binding affinity and stoichiometry is electrospray ionization mass spectrometry (ESI MS). More recent methods have also revealed the usefulness of ESI MS in determining the binding loci of small molecules on RNA. © 2019 Elsevier Inc. All rights reserved.

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