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The Use of Dried Bovine Hemoglobin and Plasma for Mass Rearing New World Screwworm.

Authors
  • Thomas, J K1
  • Fadul, G J2
  • Keller, G P3
  • Chaudhury, M F4
  • 1 USDA-APHIS-IS, U.S. Embassy Panama, Demetrio Basilio Lakas Avenue, Clayton, Panama. , (Panama)
  • 2 Panama-USA Program for the Eradication & Control of Screwworm (COPEG), Methods Development Department, Pacora, Panama. , (Panama)
  • 3 USDA-APHIS-IS, Screwworm Program, Pacora, Panama. , (Panama)
  • 4 University of Nebraska, Parducci Drive, Lincoln, NE.
Type
Published Article
Journal
Journal of Insect Science
Publisher
Oxford University Press
Publication Date
May 01, 2018
Volume
18
Issue
3
Identifiers
DOI: 10.1093/jisesa/iey052
PMID: 29878232
Source
Medline
Language
English
License
Unknown

Abstract

The success of the Screwworm Eradication Program is due to continuous mass rearing and dispersal of large numbers of competitive sterile flies in the field. Spray-dried powders of whole bovine blood, chicken egg, and milk substitute constituted the nutritional components of the traditional artificial larval diet used for mass rearing New World Screwworm (NWS), Cochliomyia hominivorax (Coquerel), Diptera: Calliphoridae. However, due to shifting availability and increasing costs of diet ingredients, it is necessary to investigate alternative products for the diet. Recently, spray-dried whole bovine blood became unavailable for purchase in the quantities that the Screwworm Program requires and thus were obliged to purchase bovine blood subproducts. Previous research showed that bovine hemoglobin could be substituted for whole blood with good results in small trials. Here, we report results of NWS larval diets prepared with bovine blood subproducts, hemoglobin and plasma, in 20-liter trays used in mass rearing. Diets were prepared using three separate hemoglobin/plasma ratios. Though all three configurations of hemoglobin and plasma were successful in the larval diet, we found the diets containing 1.5% total plasma, as opposed to 0.5 and 1%, produced heavier larvae and pupae, and resulted in more pupae per unit of diet. Considering cost, we determined that the ideal ratio for the blood portion of the diet for mass rearing is 80% hemoglobin and 20% plasma.

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