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Bioevaluation of Ranatuerin-2Pb from the Frog Skin Secretion of Rana pipiens and its Truncated Analogues.

Authors
  • Zhou, Xiaowei1, 2
  • Shi, Daning3, 4
  • Zhong, Ruimin5
  • Ye, Zhuming6
  • Ma, Chengbang7
  • Zhou, Mei8
  • Xi, Xinping9, 10
  • Wang, Lei11
  • Chen, Tianbao12
  • Kwok, Hang Fai13
  • 1 Department of Nutrition, Henry Fok School of Food Science and Engineering, Shaoguan University, Shaoguan 512005, China. [email protected] , (China)
  • 2 Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK. [email protected] , (Ireland)
  • 3 Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK. [email protected] , (Ireland)
  • 4 School of Government, Peking University, No 114, The Leo KoGuan Building, Beijing 100871, China. [email protected] , (China)
  • 5 Department of Nutrition, Henry Fok School of Food Science and Engineering, Shaoguan University, Shaoguan 512005, China. [email protected] , (China)
  • 6 Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK. [email protected] , (Ireland)
  • 7 Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK. [email protected] , (Ireland)
  • 8 Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK. [email protected] , (Ireland)
  • 9 Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK. [email protected] , (Ireland)
  • 10 Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Avenida de Universidade, Taipa, Macao. [email protected] , (Macau SAR China)
  • 11 Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK. [email protected] , (Ireland)
  • 12 Natural Drug Discovery Group, School of Pharmacy, Queen's University, Belfast BT9 7BL, Northern Ireland, UK. [email protected] , (Ireland)
  • 13 Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Avenida de Universidade, Taipa, Macao. [email protected] , (Macau SAR China)
Type
Published Article
Journal
Biomolecules
Publisher
MDPI AG
Publication Date
Jun 25, 2019
Volume
9
Issue
6
Identifiers
DOI: 10.3390/biom9060249
PMID: 31242693
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Antimicrobial peptides (AMPs) are considered as a promising agent to overcome the drug-resistance of bacteria. Large numbers of AMPs have been identified from the skin secretion of Rana pipiens, including brevinins, ranatuerins, temporins and esculentins. In this study, the cDNA precursor of a broad-spectrum antimicrobial peptide, ranatuerin-2Pb, was cloned and identified. Additionally, two truncated analogues, RPa and RPb, were synthesised to investigate the structure-activity relationship of ranatuerin-2Pb. RPa lost antimicrobial activity against Candida albicans, MRSA, Enterococcus faecalis and Pseudomonas aeruginosa, while RPb retained its broad-spectrum antimicrobial activity. Additionally, ranatuerin-2Pb, RPa and RPb demonstrated inhibition and eradication effects against Staphylococcus aureus biofilm. RPb showed a rapid bacterial killing manner via membrane permeabilization without damaging the cell membrane of erythrocytes. Moreover, RPb decreased the mortality of S. aureus infected Galleria mellonella larvae. Collectively, our results suggested that RPb may pave a novel way for natural antimicrobial drug design.

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