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Transcriptome Analyses Revealed the Key Metabolic Genes and Transcription Factors Involved in Terpenoid Biosynthesis in Sacred Lotus.

Authors
  • Qin, Lili1
  • Du, Fei1
  • Yang, Ningning1
  • Zhang, Chen1
  • Wang, Zhiwen1
  • Zheng, Xingwen2
  • Tang, Jiawei1
  • Yang, Liangbo2
  • Dong, Chen1
  • 1 College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China. , (China)
  • 2 White Lotus Industrial Development Center of Guangchang County, Fuzhou 344900, China. , (China)
Type
Published Article
Journal
Molecules
Publisher
MDPI AG
Publication Date
Jul 19, 2022
Volume
27
Issue
14
Identifiers
DOI: 10.3390/molecules27144599
PMID: 35889471
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

As the largest group of structurally diverse metabolites, terpenoids are versatile natural compounds that act as metabolism mediators, plant volatiles, and ecological communicators. However, few terpenoid compounds have been identified in plant parts of sacred lotus (Nelumbo nucifera Gaertn.). To elucidate the molecular genetic basis of the terpene biosynthetic pathway, terpenes from different parts of the plant, including seeds (S), young leaves (YL), mature leaves (ML), white flowers (WF), yellow flowers (YF), and red flowers (RF), were identified by LC-MS/MS and the relative contents of the same terpenes in different parts were compared. The results indicate that all plant parts primarily consist of triterpenes, with only minor quantities of sesquiterpenes and diterpenes, and there were differences in the terpene content detected in different plant parts. To illustrate the biosynthesis of various terpenoids, RNA sequencing was performed to profile the transcriptomes of various plant parts, which generated a total of 126.95 GB clean data and assembled into 29,630 unigenes. Among these unigenes, 105 candidate unigenes are involved in the mevalonate (MVA) pathway, methyl-erythritol phosphate (MEP) pathway, terpenoid backbone biosynthesis pathway, and terpenoid synthases pathway. Moreover, the co-expression network between terpene synthase (TPS) and WRKY transcription factors provides new information for the terpene biosynthesis pathway.

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