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Unyvero ITI® system for the clinical resolution of discrepancies in periprosthetic joint infection diagnosis.

Authors
  • Zannoli, Silvia1
  • Sambri, Andrea2, 3
  • Morotti, Manuela1
  • Tassinari, Martina1
  • Torri, Arianna1
  • Bianchi, Giuseppe4
  • De Paolis, Massimiliano4
  • Donati, Davide Maria4, 5
  • Sambri, Vittorio1, 6
  • 1 Unit of Microbiology, The Great Romagna Area Hub Laboratory, Pievesestina, Cesena, Italy. , (Italy)
  • 2 Department of Orthopaedic Oncology, Istituto Ortopedico Rizzoli, Via Pupilli 1, 40136, Bologna, Italy. [email protected] , (Italy)
  • 3 Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Bologna, Italy. [email protected] , (Italy)
  • 4 Department of Orthopaedic Oncology, Istituto Ortopedico Rizzoli, Via Pupilli 1, 40136, Bologna, Italy. , (Italy)
  • 5 Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Bologna, Italy. , (Italy)
  • 6 Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Bologna, Italy. , (Italy)
Type
Published Article
Journal
Musculoskeletal surgery
Publication Date
Apr 01, 2021
Volume
105
Issue
1
Pages
39–42
Identifiers
DOI: 10.1007/s12306-019-00626-x
PMID: 31595427
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The Unyvero molecular assay was tested for the clinical resolution of discordant results, evaluating its role in prosthetic joint infection diagnosis. Multiplex PCR was performed on 45 samples from prosthesis treatment (either sonication or dithiothreitol). Analytical performance was compared to that of biofilm culture using Musculoskeletal Infection Society criteria as gold standard. Unyvero and biofilm culture showed similar agreement rates compared to the gold standard (34/43 and 32/43, respectively). Both methods showed six additional identifications compatible with true infection; five positive results from biofilm culture were deemed contaminations. The Unyvero system showed good performances and a significantly shorter turnaround time compared to cultural methods, presenting an added value to PJI diagnosis even when performed following a composite approach.

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