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Unraveling secrets of telomeres: One molecule at a time

Authors
  • Lin, Jiangguo
  • Kaur, Parminder
  • Countryman, Preston
  • Opresko, Patricia L.
  • Wang, Hong1, 2
  • 1 Physics Department, North Carolina State University
  • 2 Department of Environmental and Occupational Health, University of Pittsburgh Graduate School of Public Health
Type
Published Article
Journal
DNA Repair
Publisher
Elsevier
Publication Date
Jan 01, 2014
Accepted Date
Jan 13, 2014
Identifiers
DOI: 10.1016/j.dnarep.2014.01.012
Source
Elsevier
Keywords
License
Unknown

Abstract

Telomeres play important roles in maintaining the stability of linear chromosomes. Telomere maintenance involves dynamic actions of multiple proteins interacting with long repetitive sequences and complex dynamic DNA structures, such as G-quadruplexes, T-loops and t-circles. Given the heterogeneity and complexity of telomeres, single-molecule approaches are essential to fully understand the structure–function relationships that govern telomere maintenance. In this review, we present a brief overview of the principles of single-molecule imaging and manipulation techniques. We then highlight results obtained from applying these single-molecule techniques for studying structure, dynamics and functions of G-quadruplexes, telomerase, and shelterin proteins.

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