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[Universal method for single nucleotide substitution identification].

Authors
  • Brenner, E V
  • Ivanova, E M
  • Pyshnyĭ, D V
  • Morozov, I V
Type
Published Article
Journal
Bioorganicheskaia khimiia
Publication Date
Jan 01, 2005
Volume
31
Issue
2
Pages
213–215
Identifiers
PMID: 15889797
Source
Medline
License
Unknown

Abstract

A new approach to the identification of point mutations by allele-specific PCR was proposed. The mutation R408W of the human phenylalanine hydroxylase gene was used as a model. A high specificity of the approach was achieved by the use of primers partially complementary to the genomic DNA. Polyethylene glycol covalently attached to one of the allele-specific primers provides for the differential identification of the PCR products due to a change in electrophoretic mobility.

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